RESUMO
Background: common use of sevoflurane in congenital defects during repeated surgeries may have detrimental effects on spermatogenesis after puberty
Objective: this study investigated sevoflurane effects on spermatogenesis process in male mature mice after exposure in prepubertal time
Materials and Methods: 24 neonatal NMRI male mice were randomly classified in three groups. Experimental 1 and 2 groups [exposure to 1 minimum alveolar concentration [MAC] and 2 MAC sevoflurane, respectively in 2 lit/min oxygen [O[2]] for 7 days [30 min, daily] and control. All groups were sacrificed after 2 months. Histological assessment, immunohistochemistry and apoptosis process was done. Bax and Bcl2 expression was evaluated in the testicular tissue by real time Poly Chain Reaction
Results: our results showed that the integrity of testicular tissue was preserved in both experimental groups. Count of spermatogonial cells had significant decrease in group 2 compared to others. The rate of apoptosis in spermatogonial cells was 15+/-3% and 9+/-2% in the group 2 and 1, respectively. Also, Bax/Bcl[2] ratio was 0.2615, 1.0070 and 9.3657 in control, experimental group 1 and 2, respectively. This result was significant [p
Conclusion: continuous exposure of 2 MAC sevoflurane in 2 lit/min O[2] simultaneous during prepubertal may create more testicular tissue damage in terms of cellular and molecular function compared to continuous exposure to lower level of sevoflurane by increase in ratio of Bax/Bcl[2] and apoptosis in germ cells after puberty